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stemcellqc  (MathWorks Inc)


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    MathWorks Inc stemcellqc
    Comparison of the morphology and dynamic features in small and large phenotype colonies. A,B, Morphology of hESCs with the small cell phenotype. C,D, Morphology of hESCs with the large cell phenotype. E,F, Neural rosettes and neurons formed from small phenotype colonies. G,H, Absence of rosettes and neurons when large phenotype colonies were incubated in neurogenesis differentiating medium. I, Solidities of large and small phenotype colonies were not significantly different. J, Number of protrusions on small and large phenotype colonies were not significantly different. K, Areas of large and small phenotype colonies were not significantly different. L, The average intensity the small and large phenotypes were significantly different between 1 and 46 hours ( P values were all <.001). M, Total displacement of large and small phenotypes was significantly different between 28 and 46 hours ( P values ranged from .05 to .001). N, Total distance traveled for small and large phenotypes became significantly different between hours 15 and 46 ( P values ranged from .05 to .0001). <t>StemCellQC</t> data are plotted as means ± the SEM of three independent experiments in which each experiment had 8‐11 colonies. A Box‐Cox transformation was applied to the average intensity data prior to performing a two‐way ANOVA with Bonferroni's post hoc test. * indicates significant differences. Scale bar = 25 μm
    Stemcellqc, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stemcellqc/product/MathWorks Inc
    Average 90 stars, based on 1 article reviews
    stemcellqc - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Video bioinformatics analysis of human pluripotent stem cell morphology, quality, and cellular dynamics"

    Article Title: Video bioinformatics analysis of human pluripotent stem cell morphology, quality, and cellular dynamics

    Journal: Stem Cells Translational Medicine

    doi: 10.1002/sctm.15-0352

    Comparison of the morphology and dynamic features in small and large phenotype colonies. A,B, Morphology of hESCs with the small cell phenotype. C,D, Morphology of hESCs with the large cell phenotype. E,F, Neural rosettes and neurons formed from small phenotype colonies. G,H, Absence of rosettes and neurons when large phenotype colonies were incubated in neurogenesis differentiating medium. I, Solidities of large and small phenotype colonies were not significantly different. J, Number of protrusions on small and large phenotype colonies were not significantly different. K, Areas of large and small phenotype colonies were not significantly different. L, The average intensity the small and large phenotypes were significantly different between 1 and 46 hours ( P values were all <.001). M, Total displacement of large and small phenotypes was significantly different between 28 and 46 hours ( P values ranged from .05 to .001). N, Total distance traveled for small and large phenotypes became significantly different between hours 15 and 46 ( P values ranged from .05 to .0001). StemCellQC data are plotted as means ± the SEM of three independent experiments in which each experiment had 8‐11 colonies. A Box‐Cox transformation was applied to the average intensity data prior to performing a two‐way ANOVA with Bonferroni's post hoc test. * indicates significant differences. Scale bar = 25 μm
    Figure Legend Snippet: Comparison of the morphology and dynamic features in small and large phenotype colonies. A,B, Morphology of hESCs with the small cell phenotype. C,D, Morphology of hESCs with the large cell phenotype. E,F, Neural rosettes and neurons formed from small phenotype colonies. G,H, Absence of rosettes and neurons when large phenotype colonies were incubated in neurogenesis differentiating medium. I, Solidities of large and small phenotype colonies were not significantly different. J, Number of protrusions on small and large phenotype colonies were not significantly different. K, Areas of large and small phenotype colonies were not significantly different. L, The average intensity the small and large phenotypes were significantly different between 1 and 46 hours ( P values were all <.001). M, Total displacement of large and small phenotypes was significantly different between 28 and 46 hours ( P values ranged from .05 to .001). N, Total distance traveled for small and large phenotypes became significantly different between hours 15 and 46 ( P values ranged from .05 to .0001). StemCellQC data are plotted as means ± the SEM of three independent experiments in which each experiment had 8‐11 colonies. A Box‐Cox transformation was applied to the average intensity data prior to performing a two‐way ANOVA with Bonferroni's post hoc test. * indicates significant differences. Scale bar = 25 μm

    Techniques Used: Incubation, Transformation Assay

    Analysis of apoptosis using the brightness/area ratio feature during culture in mTeSR1‐A. A‐D, Phase contrast image film strip of dying cells (yellow arrows) being extruded to the top of a hESC colony cultured on Matrigel. E‐P, Examples of dead cells (bright areas) on the surface of the colonies cultured on Matrigel (E‐H), Geltrex (I‐L), and laminin‐521 (M‐P). Colonies were segmented using StemCellQC (red line). Q, MitoSox Red staining of apoptotic cells on the surface of a colony. R, Trypan blue staining of cells undergoing apoptosis on the surface of a colony. S, StemCellQC brightness/area ratio analysis of colonies cultured on Matrigel, Geltrex, and laminin‐521. For brightness/area ratio data, laminin‐521 was significantly different from Matrigel between hours 1 and 46 of the experiment ( P values ranged from .05 to .001). Geltrex was significantly different from Matrigel between 28 and 48 hours ( P values ranged from .05 to .001). StemCellQC data are means ± SE of three independent experiments where each experiment had 8‐11 colonies. A Box‐Cox transformation was applied to the brightness/ratio parameter prior to performing the two‐way ANOVA. The Dunnett's post hoc test used Matrigel as a reference group. * indicates range over which means were significantly different. Scale bars: I‐O = 20 μm; Q‐R = 15 μm
    Figure Legend Snippet: Analysis of apoptosis using the brightness/area ratio feature during culture in mTeSR1‐A. A‐D, Phase contrast image film strip of dying cells (yellow arrows) being extruded to the top of a hESC colony cultured on Matrigel. E‐P, Examples of dead cells (bright areas) on the surface of the colonies cultured on Matrigel (E‐H), Geltrex (I‐L), and laminin‐521 (M‐P). Colonies were segmented using StemCellQC (red line). Q, MitoSox Red staining of apoptotic cells on the surface of a colony. R, Trypan blue staining of cells undergoing apoptosis on the surface of a colony. S, StemCellQC brightness/area ratio analysis of colonies cultured on Matrigel, Geltrex, and laminin‐521. For brightness/area ratio data, laminin‐521 was significantly different from Matrigel between hours 1 and 46 of the experiment ( P values ranged from .05 to .001). Geltrex was significantly different from Matrigel between 28 and 48 hours ( P values ranged from .05 to .001). StemCellQC data are means ± SE of three independent experiments where each experiment had 8‐11 colonies. A Box‐Cox transformation was applied to the brightness/ratio parameter prior to performing the two‐way ANOVA. The Dunnett's post hoc test used Matrigel as a reference group. * indicates range over which means were significantly different. Scale bars: I‐O = 20 μm; Q‐R = 15 μm

    Techniques Used: Stripping Membranes, Cell Culture, Staining, Transformation Assay

    StemCellQC analysis of H9 hESCs, H1 hESCs, and CC3 iPSCs. A, Solidity comparisons to H9: the CC3 cells were significantly lower during two early time intervals ( P values ranged from .05 to .001), while the H1 line was significantly different between 1 and 48 hours ( P values ranged from .05 to .001). B, Area comparisons to H9: the CC3 cells were significantly lower between 23 and 70 hours ( P values ranged from .05 to .0001), while the H1 line was significantly lower between 32 and 70 hours ( P values ranged from .05 to .0001). C‐H, Phase contrast images showing brightness on the surface of the three cell lines at the beginning and end of culture. I, Brightness/area ratio comparisons to H9: the CC3 line was significantly higher for most time points between 1‐23 and 45‐70 hours ( P values ranged from .05 to .001), while the H1 line was significantly higher between 22‐70 hours ( P values ranged from .05 to .01). J‐L, H9, H1 and CC3 colonies showing the direction and path of movement on each colony (red line). M, Total distance traveled comparisons to H9: the H1 line was significantly higher between 8 and 70 hours ( P values ranged from .05 to .0001), while the CC3 line was significantly higher between 46 and 70 hours ( P values ranged from .05 to .001). Total displacement comparisons to H9: the H1 line was significantly lower in total displacement between 15 and 70 hours ( P values ranged from .05 to .0001. * indicates significant differences. Scale bars G‐H = 15 μm; J‐L = 10 μm
    Figure Legend Snippet: StemCellQC analysis of H9 hESCs, H1 hESCs, and CC3 iPSCs. A, Solidity comparisons to H9: the CC3 cells were significantly lower during two early time intervals ( P values ranged from .05 to .001), while the H1 line was significantly different between 1 and 48 hours ( P values ranged from .05 to .001). B, Area comparisons to H9: the CC3 cells were significantly lower between 23 and 70 hours ( P values ranged from .05 to .0001), while the H1 line was significantly lower between 32 and 70 hours ( P values ranged from .05 to .0001). C‐H, Phase contrast images showing brightness on the surface of the three cell lines at the beginning and end of culture. I, Brightness/area ratio comparisons to H9: the CC3 line was significantly higher for most time points between 1‐23 and 45‐70 hours ( P values ranged from .05 to .001), while the H1 line was significantly higher between 22‐70 hours ( P values ranged from .05 to .01). J‐L, H9, H1 and CC3 colonies showing the direction and path of movement on each colony (red line). M, Total distance traveled comparisons to H9: the H1 line was significantly higher between 8 and 70 hours ( P values ranged from .05 to .0001), while the CC3 line was significantly higher between 46 and 70 hours ( P values ranged from .05 to .001). Total displacement comparisons to H9: the H1 line was significantly lower in total displacement between 15 and 70 hours ( P values ranged from .05 to .0001. * indicates significant differences. Scale bars G‐H = 15 μm; J‐L = 10 μm

    Techniques Used:



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    Comparison of the morphology and dynamic features in small and large phenotype colonies. A,B, Morphology of hESCs with the small cell phenotype. C,D, Morphology of hESCs with the large cell phenotype. E,F, Neural rosettes and neurons formed from small phenotype colonies. G,H, Absence of rosettes and neurons when large phenotype colonies were incubated in neurogenesis differentiating medium. I, Solidities of large and small phenotype colonies were not significantly different. J, Number of protrusions on small and large phenotype colonies were not significantly different. K, Areas of large and small phenotype colonies were not significantly different. L, The average intensity the small and large phenotypes were significantly different between 1 and 46 hours ( P values were all <.001). M, Total displacement of large and small phenotypes was significantly different between 28 and 46 hours ( P values ranged from .05 to .001). N, Total distance traveled for small and large phenotypes became significantly different between hours 15 and 46 ( P values ranged from .05 to .0001). <t>StemCellQC</t> data are plotted as means ± the SEM of three independent experiments in which each experiment had 8‐11 colonies. A Box‐Cox transformation was applied to the average intensity data prior to performing a two‐way ANOVA with Bonferroni's post hoc test. * indicates significant differences. Scale bar = 25 μm
    Stemcellqc, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stemcellqc/product/MathWorks Inc
    Average 90 stars, based on 1 article reviews
    stemcellqc - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

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    Comparison of the morphology and dynamic features in small and large phenotype colonies. A,B, Morphology of hESCs with the small cell phenotype. C,D, Morphology of hESCs with the large cell phenotype. E,F, Neural rosettes and neurons formed from small phenotype colonies. G,H, Absence of rosettes and neurons when large phenotype colonies were incubated in neurogenesis differentiating medium. I, Solidities of large and small phenotype colonies were not significantly different. J, Number of protrusions on small and large phenotype colonies were not significantly different. K, Areas of large and small phenotype colonies were not significantly different. L, The average intensity the small and large phenotypes were significantly different between 1 and 46 hours ( P values were all <.001). M, Total displacement of large and small phenotypes was significantly different between 28 and 46 hours ( P values ranged from .05 to .001). N, Total distance traveled for small and large phenotypes became significantly different between hours 15 and 46 ( P values ranged from .05 to .0001). StemCellQC data are plotted as means ± the SEM of three independent experiments in which each experiment had 8‐11 colonies. A Box‐Cox transformation was applied to the average intensity data prior to performing a two‐way ANOVA with Bonferroni's post hoc test. * indicates significant differences. Scale bar = 25 μm

    Journal: Stem Cells Translational Medicine

    Article Title: Video bioinformatics analysis of human pluripotent stem cell morphology, quality, and cellular dynamics

    doi: 10.1002/sctm.15-0352

    Figure Lengend Snippet: Comparison of the morphology and dynamic features in small and large phenotype colonies. A,B, Morphology of hESCs with the small cell phenotype. C,D, Morphology of hESCs with the large cell phenotype. E,F, Neural rosettes and neurons formed from small phenotype colonies. G,H, Absence of rosettes and neurons when large phenotype colonies were incubated in neurogenesis differentiating medium. I, Solidities of large and small phenotype colonies were not significantly different. J, Number of protrusions on small and large phenotype colonies were not significantly different. K, Areas of large and small phenotype colonies were not significantly different. L, The average intensity the small and large phenotypes were significantly different between 1 and 46 hours ( P values were all <.001). M, Total displacement of large and small phenotypes was significantly different between 28 and 46 hours ( P values ranged from .05 to .001). N, Total distance traveled for small and large phenotypes became significantly different between hours 15 and 46 ( P values ranged from .05 to .0001). StemCellQC data are plotted as means ± the SEM of three independent experiments in which each experiment had 8‐11 colonies. A Box‐Cox transformation was applied to the average intensity data prior to performing a two‐way ANOVA with Bonferroni's post hoc test. * indicates significant differences. Scale bar = 25 μm

    Article Snippet: StemCellQC was written and developed with the MATLAB 2015a programming environment.

    Techniques: Incubation, Transformation Assay

    Analysis of apoptosis using the brightness/area ratio feature during culture in mTeSR1‐A. A‐D, Phase contrast image film strip of dying cells (yellow arrows) being extruded to the top of a hESC colony cultured on Matrigel. E‐P, Examples of dead cells (bright areas) on the surface of the colonies cultured on Matrigel (E‐H), Geltrex (I‐L), and laminin‐521 (M‐P). Colonies were segmented using StemCellQC (red line). Q, MitoSox Red staining of apoptotic cells on the surface of a colony. R, Trypan blue staining of cells undergoing apoptosis on the surface of a colony. S, StemCellQC brightness/area ratio analysis of colonies cultured on Matrigel, Geltrex, and laminin‐521. For brightness/area ratio data, laminin‐521 was significantly different from Matrigel between hours 1 and 46 of the experiment ( P values ranged from .05 to .001). Geltrex was significantly different from Matrigel between 28 and 48 hours ( P values ranged from .05 to .001). StemCellQC data are means ± SE of three independent experiments where each experiment had 8‐11 colonies. A Box‐Cox transformation was applied to the brightness/ratio parameter prior to performing the two‐way ANOVA. The Dunnett's post hoc test used Matrigel as a reference group. * indicates range over which means were significantly different. Scale bars: I‐O = 20 μm; Q‐R = 15 μm

    Journal: Stem Cells Translational Medicine

    Article Title: Video bioinformatics analysis of human pluripotent stem cell morphology, quality, and cellular dynamics

    doi: 10.1002/sctm.15-0352

    Figure Lengend Snippet: Analysis of apoptosis using the brightness/area ratio feature during culture in mTeSR1‐A. A‐D, Phase contrast image film strip of dying cells (yellow arrows) being extruded to the top of a hESC colony cultured on Matrigel. E‐P, Examples of dead cells (bright areas) on the surface of the colonies cultured on Matrigel (E‐H), Geltrex (I‐L), and laminin‐521 (M‐P). Colonies were segmented using StemCellQC (red line). Q, MitoSox Red staining of apoptotic cells on the surface of a colony. R, Trypan blue staining of cells undergoing apoptosis on the surface of a colony. S, StemCellQC brightness/area ratio analysis of colonies cultured on Matrigel, Geltrex, and laminin‐521. For brightness/area ratio data, laminin‐521 was significantly different from Matrigel between hours 1 and 46 of the experiment ( P values ranged from .05 to .001). Geltrex was significantly different from Matrigel between 28 and 48 hours ( P values ranged from .05 to .001). StemCellQC data are means ± SE of three independent experiments where each experiment had 8‐11 colonies. A Box‐Cox transformation was applied to the brightness/ratio parameter prior to performing the two‐way ANOVA. The Dunnett's post hoc test used Matrigel as a reference group. * indicates range over which means were significantly different. Scale bars: I‐O = 20 μm; Q‐R = 15 μm

    Article Snippet: StemCellQC was written and developed with the MATLAB 2015a programming environment.

    Techniques: Stripping Membranes, Cell Culture, Staining, Transformation Assay

    StemCellQC analysis of H9 hESCs, H1 hESCs, and CC3 iPSCs. A, Solidity comparisons to H9: the CC3 cells were significantly lower during two early time intervals ( P values ranged from .05 to .001), while the H1 line was significantly different between 1 and 48 hours ( P values ranged from .05 to .001). B, Area comparisons to H9: the CC3 cells were significantly lower between 23 and 70 hours ( P values ranged from .05 to .0001), while the H1 line was significantly lower between 32 and 70 hours ( P values ranged from .05 to .0001). C‐H, Phase contrast images showing brightness on the surface of the three cell lines at the beginning and end of culture. I, Brightness/area ratio comparisons to H9: the CC3 line was significantly higher for most time points between 1‐23 and 45‐70 hours ( P values ranged from .05 to .001), while the H1 line was significantly higher between 22‐70 hours ( P values ranged from .05 to .01). J‐L, H9, H1 and CC3 colonies showing the direction and path of movement on each colony (red line). M, Total distance traveled comparisons to H9: the H1 line was significantly higher between 8 and 70 hours ( P values ranged from .05 to .0001), while the CC3 line was significantly higher between 46 and 70 hours ( P values ranged from .05 to .001). Total displacement comparisons to H9: the H1 line was significantly lower in total displacement between 15 and 70 hours ( P values ranged from .05 to .0001. * indicates significant differences. Scale bars G‐H = 15 μm; J‐L = 10 μm

    Journal: Stem Cells Translational Medicine

    Article Title: Video bioinformatics analysis of human pluripotent stem cell morphology, quality, and cellular dynamics

    doi: 10.1002/sctm.15-0352

    Figure Lengend Snippet: StemCellQC analysis of H9 hESCs, H1 hESCs, and CC3 iPSCs. A, Solidity comparisons to H9: the CC3 cells were significantly lower during two early time intervals ( P values ranged from .05 to .001), while the H1 line was significantly different between 1 and 48 hours ( P values ranged from .05 to .001). B, Area comparisons to H9: the CC3 cells were significantly lower between 23 and 70 hours ( P values ranged from .05 to .0001), while the H1 line was significantly lower between 32 and 70 hours ( P values ranged from .05 to .0001). C‐H, Phase contrast images showing brightness on the surface of the three cell lines at the beginning and end of culture. I, Brightness/area ratio comparisons to H9: the CC3 line was significantly higher for most time points between 1‐23 and 45‐70 hours ( P values ranged from .05 to .001), while the H1 line was significantly higher between 22‐70 hours ( P values ranged from .05 to .01). J‐L, H9, H1 and CC3 colonies showing the direction and path of movement on each colony (red line). M, Total distance traveled comparisons to H9: the H1 line was significantly higher between 8 and 70 hours ( P values ranged from .05 to .0001), while the CC3 line was significantly higher between 46 and 70 hours ( P values ranged from .05 to .001). Total displacement comparisons to H9: the H1 line was significantly lower in total displacement between 15 and 70 hours ( P values ranged from .05 to .0001. * indicates significant differences. Scale bars G‐H = 15 μm; J‐L = 10 μm

    Article Snippet: StemCellQC was written and developed with the MATLAB 2015a programming environment.

    Techniques: